AN OPTIMISED PIPELINE FOR PARALLEL IMAGE-BASED QUANTIFICATION OF GENE EXPRESSION AND GENOTYPING AFTER IN SITU HYBRIDISATION

An optimised pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridisation

Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines.A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH).Because the embryos can be distinguished by genotype after ISH, L-Phenylalanine comparing gene expression between wild-type and mutant siblings can be done b

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Lung recruitment in ARDS patients

GREENS CHOCOLATE Lung recruitment is used to improve oxygenation and decrease the risk of ventilator induced lung injuries.Assessing the potential of recruitability is a pre-requisite for a rational recruitment strategy and setting of PEEP.Using a low flow pressure-volume loop is helpful to assess the potential of recruitability at the bedside.For

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A mixed methods exploratory evaluation of burnout in frontline staff implementing dialectical behavior therapy on a pediatric eating disorders unit

Abstract Background Eating disorders are life-threatening illnesses that commonly affect adolescents.The treatment of individuals with eating disorders can involve slow treatment progression and addressing L-Phenylalanine comorbidities which can contribute to staff burnout.Dialectical behavior therapy (DBT) has emerged as a viable treatment option

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